Design of University Small-Scale Dairy Processing Facility

This project was assigned to research the feasibility and value of implementing a dairy processing facility on the campus of the University of Nebraska-Lincoln. The facility would process milk produced from cows under the same roof and will serve as an educational experience for Nebraska dairy farmers, UNL students, and K-12 students in the Lincoln-Lancaster County area. If the project is successful and replicated across the state, this facility could have a significant impact on the reduction of milk transportation costs in the Nebraska dairy industry. The project began with researching milk processing methods and steps from production to consumption. Shortly after this step, information on milk consumption patterns was collected from UNL Dining Services to determine demand on campus. Every unit operation requires certain equipment to effectively ensure the safety and quality of the final product, and mass balances from UNL milk consumption data were used to size equipment and storage capacity. Engineering firms were then consulted to gather information on equipment specifications and prices. Equipment costs and operating costs (estimated with the help of Dr. Howell and other university dairy operations) were entered into a Monte Carlo simulation to analyze return on investment and a breakeven point. The results from the costs section showed that the fixed costs (equipment and engineering) for the milk processing would be about $1.2 million. The Monte Carlo simulation showed that the project would not turn a profit for 10-12 years, and approximately 2.25 million gallons of milk would need to be processed and sold to recover initial costs. Overall, the project successfully displays data that can be interpreted by the client to decide whether to move forward with the project and the appropriate scale for the project at UNL

Spitzer Mid-Infrared Spectroscopy of Infrared Luminous Galaxies at z~2 III: Far-IR to Radio Properties and Optical Spectral Diagnostics

We present the far-IR, millimeter, and radio photometry as well as optical and near-IR spectroscopy of a sample of 48 z~1-3 Spitzer-selected ULIRGs with IRS mid-IR spectra. Our goals are to compute their bolometric emission, and to determine both the presence and relative strength of their AGN and starburst components. We find that strong-PAH sources tend to have higher 160um and 1.2mm fluxes than weak-PAH sources. The depth of the 9.7um silicate feature does not affect MAMBO detectability. We fit the far-IR SEDs of our sample and find an average ~7x10^{12}Lsun for our z>1.5 sources. Spectral decomposition suggests that strong-PAH sources typically have ~20-30% AGN fractions. Weak-PAH sources by contrast tend to have >~70% AGN fractions, with a few sources having comparable contributions of AGN and starbursts. The optical line diagnostics support the presence of AGN in the bulk of the weak-PAH sources. With one exception, our sources are narrow-line sources, show no obvious correspondence between the optical extinction and the silicate feature depth, and, in two cases, show evidence for outflows. Radio AGN are present in both strong-PAH and weak-PAH sources. This is supported by our sample's far-IR-to-radio ratios (q) being consistently below the average value of 2.34 for local star-forming galaxies. We use survival analysis to include the lower-limits given by the radio-undetected sources, arriving at =2.07+/-0.01 for our z>1.5 sample. In total, radio and, where available, optical line diagnostics support the presence of AGN in 57% of the z>1.5 sources, independent of IR-based diagnostics. For higher-z sources, the AGN luminosities alone are estimated to be >10^{12}Lsun, which, supported by the [OIII] luminosities, implies that the bulk of our sources host obscured quasars.Comment: 22 pages, 14 figures, accepted for publication in Ap

An evolutionary and structural characterization of mammalian protein complex organization

Background: We have recently released a comprehensive, manually curated database of mammalian protein complexes called CORUM. Combining CORUM with other resources, we assembled a dataset of over 2700 mammalian complexes. The availability of a rich information resource allows us to search for organizational properties concerning these complexes. Results: As the complexity of a protein complex in terms of the number of unique subunits increases, we observed that the number of such complexes and the mean non-synonymous to synonymous substitution ratio of associated genes tend to decrease. Similarly, as the number of different complexes a given protein participates in increases, the number of such proteins and the substitution ratio of the associated gene also tend to decrease. These observations provide evidence relating natural selection and the organization of mammalian complexes. We also observed greater homogeneity in terms of predicted protein isoelectric points, secondary structure and substitution ratio in annotated versus randomly generated complexes. A large proportion of the protein content and interactions in the complexes could be predicted from known binary protein-protein and domain-domain interactions. In particular, we found that large proteins interact preferentially with much smaller proteins. Conclusions: We observed similar trends in yeast and other data. Our results support the existence of conserved relations associated with the mammalian protein complexes

BLAST05: Power Spectra of Bright Galactic Cirrus at Submillimeter Wavelengths

We report multi-wavelength power spectra of diffuse Galactic dust emission from BLAST observations at 250, 350, and 500 microns in Galactic Plane fields in Cygnus X and Aquila. These submillimeter power spectra statistically quantify the self-similar structure observable over a broad range of scales and can be used to assess the cirrus noise which limits the detection of faint point sources. The advent of submillimeter surveys with the Herschel Space Observatory makes the wavelength dependence a matter of interest. We show that the observed relative amplitudes of the power spectra can be related through a spectral energy distribution (SED). Fitting a simple modified black body to this SED, we find the dust temperature in Cygnus X to be 19.9 +/- 1.3 K and in the Aquila region 16.9 +/- 0.7 K. Our empirical estimates provide important new insight into the substantial cirrus noise that will be encountered in forthcoming observations.Comment: Submitted to the Astrophysical Journal. Maps and other data are available at http://blastexperiment.info

A panchromatic study of BLAST counterparts: total star-formation rate, morphology, AGN fraction and stellar mass

We carry out a multi-wavelength study of individual galaxies detected by the Balloon-borne Large Aperture Submillimeter Telescope (BLAST) and identified at other wavelengths, using data spanning the radio to the ultraviolet (UV). We develop a Monte Carlo method to account for flux boosting, source blending, and correlations among bands, which we use to derive deboosted far-infrared (FIR) luminosities for our sample. We estimate total star-formation rates for BLAST counterparts with z < 0.9 by combining their FIR and UV luminosities. Star formation is heavily obscured at L_FIR > 10^11 L_sun, z > 0.5, but the contribution from unobscured starlight cannot be neglected at L_FIR < 10^11 L_sun, z < 0.25. We assess that about 20% of the galaxies in our sample show indication of a type-1 active galactic nucleus (AGN), but their submillimeter emission is mainly due to star formation in the host galaxy. We compute stellar masses for a subset of 92 BLAST counterparts; these are relatively massive objects, with a median mass of ~10^11 M_sun, which seem to link the 24um and SCUBA populations, in terms of both stellar mass and star-formation activity. The bulk of the BLAST counterparts at z<1 appear to be run-of-the-mill star-forming galaxies, typically spiral in shape, with intermediate stellar masses and practically constant specific star-formation rates. On the other hand, the high-z tail of the BLAST counterparts significantly overlaps with the SCUBA population, in terms of both star-formation rates and stellar masses, with observed trends of specific star-formation rate that support strong evolution and downsizing.Comment: Accepted for publication in the Astrophysical Journal. 44 pages, 11 figures. The SED template for the derivation of L_FIR has changed (added new figure) and the discussion on the stellar masses has been improved. The complete set of full-color postage-stamps can be found at http://blastexperiment.info/results_images/moncelsi

Observations of Arp 220 using Herschel-SPIRE: An Unprecedented View of the Molecular Gas in an Extreme Star Formation Environment

We present Herschel SPIRE-FTS observations of Arp~220, a nearby ULIRG. The FTS continuously covers 190 -- 670 microns, providing a good measurement of the continuum and detection of several molecular and atomic species. We detect luminous CO (J = 4-3 to 13-12) and water ladders with comparable total luminosity; very high-J HCN absorption; OH+, H2O+, and HF in absorption; and CI and NII. Modeling of the continuum yields warm dust, with T = 66 K, and an unusually large optical depth of ~5 at 100 microns. Non-LTE modeling of the CO shows two temperature components: cold molecular gas at T ~ 50 K and warm molecular gas at T ~1350 K. The mass of the warm gas is 10% of the cold gas, but dominates the luminosity of the CO ladder. The temperature of the warm gas is in excellent agreement with H2 rotational lines. At 1350 K, H2 dominates the cooling (~20 L_sun/M_sun) in the ISM compared to CO (~0.4 L_sun/M_sun). We found that only a non-ionizing source such as the mechanical energy from supernovae and stellar winds can excite the warm gas and satisfy the energy budget of ~20 L_sun/M_sun. We detect a massive molecular outflow in Arp 220 from the analysis of strong P-Cygni line profiles observed in OH+, H2O+, and H2O. The outflow has a mass > 10^{7} M_sun and is bound to the nuclei with velocity < 250 km/s. The large column densities observed for these molecular ions strongly favor the existence of an X-ray luminous AGN (10^{44} ergs/s) in Arp 220.Comment: Accepted in ApJ on September 1, 201

Definition of the viral targets of protective HIV-1-specific T cell responses

<p>Abstract</p> <p>Background</p> <p>The efficacy of the CTL component of a future HIV-1 vaccine will depend on the induction of responses with the most potent antiviral activity and broad HLA class I restriction. However, current HIV vaccine designs are largely based on viral sequence alignments only, not incorporating experimental data on T cell function and specificity.</p> <p>Methods</p> <p>Here, 950 untreated HIV-1 clade B or -C infected individuals were tested for responses to sets of 410 overlapping peptides (OLP) spanning the entire HIV-1 proteome. For each OLP, a "protective ratio" (PR) was calculated as the ratio of median viral loads (VL) between OLP non-responders and responders.</p> <p>Results</p> <p>For both clades, there was a negative relationship between the PR and the entropy of the OLP sequence. There was also a significant additive effect of multiple responses to beneficial OLP. Responses to beneficial OLP were of significantly higher functional avidity than responses to non-beneficial OLP. They also had superior in-vitro antiviral activities and, importantly, were at least as predictive of individuals' viral loads than their HLA class I genotypes.</p> <p>Conclusions</p> <p>The data thus identify immunogen sequence candidates for HIV and provide an approach for T cell immunogen design applicable to other viral infections.</p

BLAST: A Far-Infrared Measurement of the History of Star Formation

We directly measure redshift evolution in the mean physical properties (far-infrared luminosity, temperature, and mass) of the galaxies that produce the cosmic infrared background (CIB), using measurements from the Balloon-borne Large Aperture Sub-millimeter Telescope (BLAST), and Spitzer which constrain the CIB emission peak. This sample is known to produce a surface brightness in the BLAST bands consistent with the full CIB, and photometric redshifts are identified for all of the objects. We find that most of the 70 micron background is generated at z <~ 1 and the 500 micron background generated at z >~ 1. A significant growth is observed in the mean luminosity from ~ 10^9 - 10^12 L_sun, and in the mean temperature by 10 K, from redshifts 0< z < 3. However, there is only weak positive evolution in the comoving dust mass in these galaxies across the same redshift range. We also measure the evolution of the far-infrared luminosity density, and the star-formation rate history for these objects, finding good agreement with other infrared studies up to z ~1, exceeding the contribution attributed to optically-selected galaxies.Comment: Accepted for publication in the Astrophysical Journal. Maps available at http://blastexperiment.info

Evidence for Environmental Changes in the Submillimeter Dust Opacity

The submillimeter opacity of dust in the diffuse Galactic interstellar medium (ISM) has been quantified using a pixel-by-pixel correlation of images of continuum emission with a proxy for column density. We used three BLAST bands at 250, 350, and 500 \mu m and one IRAS at 100 \mu m. The proxy is the near-infrared color excess, E(J-Ks), obtained from 2MASS. Based on observations of stars, we show how well this color excess is correlated with the total hydrogen column density for regions of moderate extinction. The ratio of emission to column density, the emissivity, is then known from the correlations, as a function of frequency. The spectral distribution of this emissivity can be fit by a modified blackbody, whence the characteristic dust temperature T and the desired opacity \sigma_e(1200) at 1200 GHz can be obtained. We have analyzed 14 regions near the Galactic plane toward the Vela molecular cloud, mostly selected to avoid regions of high column density (N_H > 10^{22} cm^-2) and small enough to ensure a uniform T. We find \sigma_e(1200) is typically 2 to 4 x 10^{-25} cm^2/H and thus about 2 to 4 times larger than the average value in the local high Galactic latitude diffuse atomic ISM. This is strong evidence for grain evolution. There is a range in total power per H nucleon absorbed (re-radiated) by the dust, reflecting changes in the interstellar radiation field and/or the dust absorption opacity. These changes affect the equilibrium T, which is typically 15 K, colder than at high latitudes. Our analysis extends, to higher opacity and lower T, the trend of increasing opacity with decreasing T that was found at high latitudes. The recognition of changes in the emission opacity raises a cautionary flag because all column densities deduced from dust emission maps, and the masses of compact structures within them, depend inversely on the value adopted.Comment: Original version (22 Dec 2011): 14 pages, 8 figures. Revised version (24 February 2012) accepted for publication in the Astrophysical Journal (14 March 2012): elaborated details of analysis, extended discussion including new Appendix; abstract, results, conclusions unchanged. 16 pages, 9 figure

Initial conditions for star formation in clusters: physical and kinematical structure of the starless core Oph A-N6

We present high spatial (<300 AU) and spectral (0.07 km/s) resolution Submillimeter Array observations of the dense starless cluster core Oph A-N6, in the 1 mm dust continuum and the 3-2 line of N2H+ and N2D+. The dust continuum observations reveal a compact source not seen in single-dish observations, of size ~1000 AU and mass 0.005-0.01 M\odot. The combined line and single-dish observations reveal a core of size 3000 \times 1400 AU elongated in a NW-SE direction, with almost no variation in either line width or line center velocity across the map, and very small non-thermal motions. The deuterium fraction has a peak value of ~0.15 and is >0.05 over much of the core. The N2H+ column density profile across the major axis of Oph A-N6 is well represented by an isothermal cylinder, with temperature 20 K, peak density 7.1 \times 10^6 cm^{-3}, and N2H+ abundance 2.7 \times 10^{-10}. The mass of Oph A-N6 is estimated to be 0.29 M\odot, compared to a value of 0.18 M\odot from the isothermal cylinder analysis, and 0.63 M\odot for the critical mass for fragmentation of an isothermal cylinder. Compared to isolated low-mass cores, Oph A-N6 shows similar narrow line widths and small velocity variation, with a deuterium fraction similar to "evolved" dense cores. It is significantly smaller than isolated cores, with larger peak column and volume density. The available evidence suggests Oph A-N6 has formed through the fragmentation of the Oph A filament and is the precursor to a low-mass star. The dust continuum emission suggests it may already have begun to form a star.Comment: 42 pages, 12 figures, accepted by Ap